The effect of pH, temperature and divalent metalion on the extraction and agglutination of the golden snail (Pomacea sp.) lectin

Date

3-1997

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Florinia B. Merca

Co-adviser

Cleofe A. Calanasan

Committee Member

Antonio C. Laurena

Abstract

The effect of pH and temperature on the extraction of golden snail, Pomacea sp. lectin was determined The following pH values: 6.2, 7.2, 8.2 and temperatures: 4°C, 28°C, 35°C were used Results showed that temperature did not affect the extraction of the protein, and the optimum pH for extraction of the lectin was at pH 6.2. The effects of pH, temperature and divalent metal ion on the agglutination of Golden Snail lectin were also determined. The pH and temperature values that were used were the same as that in extraction The divalent metal ions used were Ba", Ca' and Mg* Agglutination was found to be highly affected by temperature Cold temperature, 4°C, increased lectin activity while higher temperature lowers the activity Mildly acidic to mildly basic pH did not affect lectin activity: Ca' significantly increased the activity of golden snail lectin while Ba' and Mg' • showed no effect The non-blood type specificity of golden snail lectin was found to be independent of pH, temperature and divalent metal ions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1997 C4 G66

Document Type

Thesis

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