Enzymatic extraction of essential oil from calamansi rind (Citrus microcarpa bunge) using pectinase from Aspergillus sp.

Date

3-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Co-adviser

Nieva T. Librojo-Basilio

Committee Member

Marivic S. Lacsamana

Abstract

Essential oil from the rind of calamansi (Citrus microcarpa Bunge) was extracted using pectinate. Maximization of essential oil yield was done by optimizing conditions such as enzyme concentration, incubation time, incubation temperature and sample:buffer dilution ratio. The optimized conditions for the enzymatic extraction of essential oil from calamansi rind were as follows: enzyme concentration, 0.50% (w/v); incubation time, 8 hours; incubation temperature, room temperature (-30°C) and sample:buffer dilution ratio, 1:2 (w/v). Using the optimized conditions, essential oil yields of 0.91% and 2.02% were obtained for the untreated (control) and pectic enzyme treated calamansi rind respectively.. Pectic enzyme treatment resulted in a 2.22 fold increase in the calamansi oil yield. Physical and chemical analyses from essential oil of untreated calamansi rind showed that it was a greenish yellow, clear oily liquid with specific gravity of 0.727 and refractive index of 1.4352. On the other hand, the essential oil of the enzyme treated calamansi rind was a brownish yellow, clear oily liquid with specific gravity of 0.707 and refractive index of 1.4150. Both untreated and pectinase treated calamansi oils were slightly soluble in 95% ethanol at 1:1 (v/v). Chromatographic analyses performed on essential oils from the untreated (control) and enzyme treated calamansi rind showed three and four peaks, respectively. The major component identified for both calamansi oils was limonene with concentrations of 92.77% and 97.73% for the untreated (control) and pectic enzyme treated respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 C4 I23

Document Type

Thesis

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