Development of rapid method for the detection of phenylurea herbicide residues in water and plant samples (apple)

Date

3-1997

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Amelia W. Tejada

Co-adviser

Carlito R. Barril

Committee Member

Lilia S. P. Madamba

Abstract

A rapid method for the detection of phenylurea herbicides was developed using colorimetric techniques. Standard solution of phenylurea herbicide is hydrolyzed with 4N HCl and then converted into a diazonium salt with NaN0/. The diazonium salt is then transformed into an azo dye using 2% N-(1-naphthyl)-ethylenediamine dihydrochloride solution. The colorimetric technique was then developed and packaged into a rapid field test kit for detection of phenylurea herbicides. This consists of alcohol lamp, volumetric flask, funnel, 95% ethanol, 4N HCI, 1% NaNO2, 10% sulfamic acid, and 2% N-(1 -naphthyl)- ethylenediamine dihydrochloride. The analysis is completed in 30 minutes. The developed technique was applied in the analysis of water and plant samples. A plant sample (peels of apple) was spiked with different concentration of diuron, a type of phenylurea herbicides, and subjected to the same technique. The limit of detection of the devised method for diuron is 0.4 ug / 5 ml H2O and 0.28 pig / 5g peels of apple.. The % recovery of diuron from the plant sample using 95% ethanol as extracting solvent ranged from 69-71%. Possible interferences were also investigated.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1997 C4 J38

Document Type

Thesis

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