Isolation, purification, and partial characterization of a lectin from the leaves of Schefflera odorata (Blanco) Merr. & Rolfe

Date

6-2000

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Co-adviser

Jose Santos

Committee Member

Florinia E. Merca

Abstract

Leaves of twelve locally available medicinal plant species were surveyed for lectin activity. Only three of these plant species namely Mangifera indica (mangga), Impatiens balsamina (kamantigue) and Schefflera odorwa (lima-lima) showed positive agglutination toward slightly outdated human blood types A, B, AB, and 0. The crude leaf extract of Schefflera adorata showed the strongest agglutination activity thus, it was subjected to isolation and further purification using ammonium sulfate fractionation and gel chromatography on Sephadex G-I50 The purification procedure employed resulted in a 2-fold purification for human blood types A, B and 0 and a 5-fold purification for blood type AB Polyacrylamide gel electrophoresis under non-denaturing conditions of the purified lectin gave a single band. The lectin was found to be both non-blood type and non-blood group specific since it agglutinated the erythrocytes from human blood types A, B, AB and 0 and those from carabao, cattle and goat. The carbohydrate specificity of the lectin was not directed towards any of the sugars tested. The purified lectin was observed to be a glycoprotein containing 2.33% total sugars. SDS-PAGE analysis of the purified lectin revealed a single band and its estimated molecular weight is 217 kDa

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2000 C4 K35

Document Type

Thesis

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