Isolation and partial characterization of molluscicidal constituents from the seeds of Barringtonia asiatica (Linnaeus) kurz.

Date

4-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Florinia E. Merca

Co-adviser

Cristina P. Bajet

Committee Member

Elmer Rico E. Mojica

Abstract

Extracts of mature seeds of Barringtonia asiatica were evaluated for its molluscicidal activity. The ground seeds were extracted sequentially with solvents of decreasing polarity using water, ethanol, and ethyl acetate. The extracts were subjected to molluscicidal assay using golden apple snail, Pomacea canaliculata. Ethyl acetate extract was found to be the most active with LC. of 100 ppm and an LC50 of 25.4 ppm, followed by water extract (LC. =100ppm, LC100 = 36.0ppm), then ethanol extract (LC100 = 200 ppm, LC5o = 96.4ppm). The water extract was partitioned through liquid- liquid extraction with n-butanol. The ethyl acetate extract was fractionated by Vacuum Liquid Chromatography (VLC) using petroleum ether and ethyl acetate resulting to three fractions (EM, EA2, and EA3). Ethanol extract was also fractionated through VLC using combinations of dichloromethane and ethanol as the eluting solvent. Three fractions (El, E2, and E3) were obtained. All fractions were assayed for molluscicidal activity at 100 ppm concentration for 24 hours observation period. The butanol extract (B) was found to be the most active with 95% mortality, followed by EA3 (85%), EA2 (55%), El (35%), A (10%), and E3 (5%). The froth test detects the presence of saponins in water and ethanol extracts but not in ethyl acetate extract The most active fractions. B and EA3, were characterized through solubility tests and series of chemical tests for functional groups. Based from the results, B is possibly a water soluble saponin glycoside or an alkaloid while EA3 is possibly a rotenoid or steroid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 C4 L36

Document Type

Thesis

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