Phenolic compounds in "Tambleng"(Cocci nia grandis L. Voight) leaves: profile, levels antioxidant and anti-inflammatory activities

Date

10-2010

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Abstract

The profile, levels, antioxidant and anti-inflammatory activities of "tamleng" (Coccinia grandis L. Voight) leaves were determined. Extraction was performed by using methanol-acetone-water (MAW 7:7:2, v/v/v) as extracting solvent. Diethyl ether was used to wash the extract in order to remove chlorophyll which could interfere in analysis. The MAW extract was further hydrolyzed with acid to release the phenolic compounds from their bound form. Isolation of the major phenolic compounds present in the extracts was done by using micro-crystalline TLC. The isolated compounds in the MAW extract were p-coumaric acid-O-glycoside and sinapic acid-O-glycoside, while those of the acid-hydrolyzed were p-coumaric acid and sinapic acid. The total phenolic content of the MAW extract was determined using the Folin-Ciocalteau reagent. Results showed 43.55 mg GAE/100 g fresh sample and 58.88 mg QE/100 g fresh sample. The MAW extract was further evaluated for its antioxidant activity through the DPPI-I Assay. Quercetin and ferulic acid were used for parallel study. Results showed Quercetin as the most effective free-radical scavenger with an IC50 of 2.99 ppm, followed by ferulic acid with an IC50 of 11.46 ppm. The MAW extract showed an IC50 at 94.59 ppm. An in vivo duck embryo CAM irritation assay was also performed on the MAW extract. Quercetin, ferulic acid and hydrocortisone were used for parallel study. The MAW extract exhibited anti-inflammatory activity comparable to that of hydrocortisone. Based on the results, "tamleng" leaves contain significant levels of phytochemicals which exert antioxidant and anti-inflammatory activities which when incorporated in the diet, could improve human health.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2010 C42 L48

Document Type

Thesis

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