Isolation and molecular weight determination of albumin subunits from makapuno nut endosperm

Date

6-1992

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

Albumin fraction of makapuno nut endosperm was extracted using prechilled distilled water. The protein was precipitated by adding 1 N HCL at pH 3.9. Gel chromatography on a Sephadex G-75 and G-100 column using 20 mM sodium phosphate buffer yielded two peaks with the peaks in G-100 relatively smaller. SDS-polyacrylamide gel electrophoresis of the albumin fraction revealed three bands (Rm - 0.94, 0.79, 0.08) with corresponding molecular weights of 14,000, 23,000 and 59,000 daltons.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1992 C4 L56

Document Type

Thesis

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