Isolation, characterization and molluscicidal evaluation of the constituents of mature wild sunflower leaves Tithonia diversifolia (Hemsl.) A. Gray

Jay R. Lomeda

Date

10-2000

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Co-adviser

Evamarie P. Capareda

Committee Member

Maxima E. Flavier

Abstract

Crude extracts from wild sunflower leaves were obtained by sequential extraction using solvents of increasing polarity, namely hexanes, ethyl acetate and methanol. Molluscicidal activity of the extracts was tested by snail contact toxicity assay. The ethyl acetate crude extract showed 100% mortality at 250 ppm. Bioassay-guided chromatographic fractionation of the ethyl acetate extract led to the isolation of a white crystalline solid, JRL, with a melting point range of 62-65°C. The isolate came from fraction CA which exhibited 100% mortality at 100 ppm. Spectroscopic analyses. namely. IR, UV, "C and '11 spectroscopy, indicated that JRL has a saturated lactone skeleton with alcohol and ether functional groups.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2000 A13 L66

Document Type

Thesis

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