Determination of extractable iron in kulitis (Amaranthus spinosus), sili-silihan (Cleome rutidosperma) and malunggay (Moringa oleifera)

Date

4-2003

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teofila DC Villar

Co-adviser

Maxima E. flavier

Abstract

The water extractable iron content of Amaranth. spinosus (kulitis), Cleome rutidosperma and Moringa oleifera (malunggay) was determined. The total iron content of the plant samples determined by Flame Atomic Absorption Spectrophotometry (FAAS) were 209.35 ± 15.76 mg/kg for A. spinosus, 202.88 ± 24.59 mg/kg for C. rutidosperma and 270.24 ± 2.38 mg/kg for M oleifera, fresh weight basis. Differences were accounted to species variation. Determination of extractable iron was done using a specific reaction between Fe' and a, a'- dipyridyl solution. To evaluate this colorimetric method, recovery assays and detection of possible matrix interference were conducted. The precision of the instrument with regards to iron determination was also evaluated. Mean levels of ferrous iron were higher for the fresh samples compared to the dried samples. Calculated values in mg/kg fresh weight basis for the fresh samples were 4.98 ± 0.48, 3.13 ± 0.95 and 10.92 ± 1.67 for A. spinosus, C. rutidosperma and Moleifera, respectively. For the dried samples, values of ferrous iron in mg/kg fresh weight basis were 2.89 ± 0.67, 2.94 ± 0.75 and 6.81 ± 1.45 for A. spinosus, C. rutidosperma and M oleifera, respectively. Levels of total iron in the extracts determined by FAAS were consistently higher and significantly different compared to total iron determined by UVVis Spectrophotometry for both treatments. Analysis of the pellets and residue by FAAS showed that iron extracted may have been trapped physically in the pellet during centrifugation. Results of stability study showed that storing the extracts at refrigerated temperature slowed down the oxidation of ferrous iron to ferric iron. Future studies could be geared up towards improving the extraction procedure and sample handling to avoid losses of iron in the samples.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2003 A13 M34

Document Type

Thesis

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