HPLC analysis of ascorbic acid content in calamansi, Citrus madurensis Lour., citrus fruit

Date

11-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose S. Solis

Abstract

HPLC, like other chromatographic methods, does not only allow separation of chemical species in a mixture but also quantitatively determine their concentrations. Position of the peaks in a chromatogram is used for identification, retention time is for qualitative analysis, and peak areas and peak heights are for quantitative analysis. Reverse-phase chromatography was applied to calamansi, Citrus madurensis Lour., citrus fruits. The ascorbic acid content of the samples was established as to the different sources or areas in the Philippines (Cabanatuan City, Baguio City and Ilocos Norte). External calibration method was employed and plots of peak area and peak height versus analyte concentration gave good linearity (R2 = 0.9644 to 1.000). Results obtained for both standards and samples were precise with %RSD generally lower than 5.0%. The efficiencies of three extraction methods namely; manual extraction, mechanical extraction and automatic extraction, were analyzed and compared based on the ascorbic acid content of the samples. From the calculated results, it was found out that manual extraction is the best and most effective process for the extraction of calamansi juice. The average AA concentration of the samples ranges from 40.0 ppm (automatic extraction) to 86.3 ppm (manual extraction). Furthermore, the sample which was obtained from !lows Norte has the highest AA concentration (76.9 ppm) using both peak area and peak height.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2005 C4 M36

Document Type

Thesis

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