Isolation and partial characterization of an insecticidal constituent of Blumea balsamifera (Linn.) DC

Date

10-1995

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. Capareda

Abstract

The CH2Cl2 extract of Blunea balsarnifera (Linn.) leaves was found to be insecticidal against Aphis craccivora (black bean aphid) adults and third-instar Spodoptera litura (common cutworm) larvae. Bioassay-directed isolation of the insecticidal component was achieved by flash column chromatography and radial thin layer chromatography. One pure compound was isolated and partially characterized. Percent mortalities were determined using the residual exposure or contact method and evaluated by one-way analysis of variance (ANOVA). The activity of the purified isolate (0.007% of dry weight of leaves) was compared with that of the insecticide Azodrin (monocrotophos). Results indicated that the isolate was more effective than the control, but less effective than Azodrin at equivalent dosages. The isolated compound had the following physical properties: color is greenish yellow; [a] 34D, +0.18° (c = 0.05, CH3COCH3) ; and UV , 231 run (in CH2Cl2). It is insoluble in water but soluble in most organic solvents of medium to high polarity. The alkaloidal nature of the isolated compound was apparent from its infrared spectrum and its positive reaction with Mayer's reagent.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1995 C4 M37

Document Type

Thesis

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