Batch production of alpha- amylase by Bacillus subtilis NRRL B3411 in airlift and stirred-tank fermenters

Date

3-1986

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

Alpha-amylase enzyme was produced in an airlift fermenter by Bacillus subtilis N13131, B3411 in a batch process using cassava as carbon source, rice bran as i nitrogen source and supplemented with peptone. Optimal conditions for enzyme production were determined namely pH, aeration rate and fermentation time. The productivity was compared at 40°C and at room temperature (27-31°C). Maximal enzyme yields were obtained at pH 7.0, 1.0 vvm aeration rate, and after the second day of fermentation at 40°C. Room temperature was found to give optimum yields at pH 6.5, after the third day of fermentation using 1.0 vvm aeration rate.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1986 C4 M37

Document Type

Thesis

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