Isoelectric precipitation of the leaf lectin from Schefflera odorata ( Blanco) Merr. & Rolfe and its effect on symptom development of bacterial leaf blight of rice

Date

4-2011

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Abstract

A lectin from the leaves of Schefflera odorata (Blanco) Merr. & Rolfe was extracted with the use of 0.02 M PBS (pH 7.2,0.15 M Noel) followed by isoelectric precipitation at pH 8.08. The leaf lectin showed a single band when subjected to both native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate PAGE (SDS-PAGE). It has an estimated molecular weight of 196 kDa and was found to be non-blood type specific when tested with human blood types A, B, and 0. It also did not exhibit specificity towards D(+)-galactose, D(+)-glucose, inositol, a-lactose. D-mannitol, mannose. melbiose. sucrose, sorbitol and D(+)-xylose. The effect of the isolated leaf lectin on symptom development of bacterial leaf blight of rice caused by Xanthomonas oryzae pv oryzae (Xoo) was investigated by application of lectin (1000 ppm) on the rice plant leaves using the clipping method and foliar spraying at one hour and at one day prior to the inoculation of the bacteria. Reductions in lesion length using the clipping method and in the diseased leaf area using the foliar spray method were observed in the samples applied with the lectin one hour prior to inoculation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 A13 M37

Document Type

Thesis

This document is currently not available here.

Share

COinS