Extraction and isolation of protein from plant leaves

Date

1971

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lilia S. P. Madamba

Abstract

EXTRACTION AND ISOLATION OF PROTEIN FROM PLANT LEAVES

OLIVIA D NARCISO

ABSTRACT

The nitrogenous constituents were extracted and isolated from leaves of four different plant species, namely) Cucharita (red var.) (Alternanthera versicolor Regel), Katuray (Sesbania orandiflora (L.) Pers.), Nalunggay (Moringa oleifera), and Pinkapinkahan (Oroxylum indicum (L.) Vent.).

Water extracts of the leaves were prepared, the pH adjusted to 4.5, and the protein coagulated by heating at 60-70°C. Extractable nitrogen and protein-nitrogen values were determined.

Katuray had the highest extractable protein-nitrogen value (25.40%), followed by Pinkapinkahan (17.01%). Low protein extraction of values were obtained from Cucharita and Malunggay leaves.

Four methods of drying were tried, namely, modified air-drying method, drying by the use of an infra-red lamp, vacuum oven drying and freeze drying. Freeze drying was found to be the best method for drying crude protein isolates.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1971 A13 N37

Document Type

Thesis

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