Isolation and characterization of the non-cellulosic polysaccharides from alibangbang seeds (Piliostigma malabarica)

Date

10-1996

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maxima E. Flavier

Abstract

ABSTRACT Ortiz, Vermilyn Norte. University of the Philippines at Los Banos. October, 1996. Isolation and Characterization of Non-Cellulosic Polysaccharides from Alibangbang Seeds (Piliostiama maiabariaa)

Adviser: Dr. Maxima E. Flavier

Proximate analysis of the whole Alibangbang seeds and the endosperm showed high crude protein content (34.39% and 35.64%), crude fat (22.27% and 27.80%), as well as, nitrogen-free extract (32.95% and 30.74%). The starch content was 9.63% (whole seed) and 8.62% (endosperm) while the total soluble sugars were 6.42% (whole seed) and 8.27% (endosperm). The whole seed and the seed endosperm (without seedcoat) were sequentially fractionated using hot water (50°C), 2.0M KOH and 2.0M HaOH. The results of fractionation showed that the alkali soluble materials represent the major fractions for the whole seed and the endosperm. The KOH-soluble material constitutes the major fraction (33.70%-40.94%) of the whole seed whereas the endosperm is comprised mostly of NaOH-soluble polymers (21.08%- 25.70%). The monosaccharides of the whole seed, endosperm, and their fractions were analyzed as alditol acetates by capillary gas chromatography. Results showed that glucose, arabinose and xylose were the predominant sugars in all samples and their fractions with traces of rhamnose, mannose, and galactose.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1996 A13 O78

Document Type

Thesis

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