Immobilization of yeast invertase using chitosan as solid support for the bench scale inversion of sucrose

Date

4-2001

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Veronica C. Sabularse

Abstract

PANGANIBAN, THERESA MARGARET DE LUNA. University of the Philippines Los Banos, April 2001. Immobilization of yeast invertase using chitosan as solid sunned for the bench scale inversion of sucrose.

Adviser: Dr. Veronica C. Sabularse

Yeast invertase was immobilized with chitosan as solid support. Chitosan was allowed to react with glutaraldehyde to provide sites for invertase attachment. Reaction of the chitosan-glutaraldehyde with 2,4-dinitrophenylhydrazine and Benedict's solution indicated the attachment of glutaraldehyde to chitosan.

Laboratory and bench scale columns were prepared and compared for efficiency of inversion. Statistical analysis showed that bench scale inversion was almost 40% higher, in terms of both activity and specific activity, than laboratory scale inversion.

The optimum sucrose concentration was found to be 0.3 M. Enzyme activity retained after storage and intermittent use over a period of six weeks was 88% for the laboratory scale and 83% for the bench scale column. Statistical analysis showed that there were no differences at a 5% level of significance in the specific activities of the enzyme for six weeks. The stability of the enzyme towards continuous use for a period of twelve hours indicated a relatively rapid increase in activity during the first six hours and a gradual increase after eight hours.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2001 C4 P36

Document Type

Thesis

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