Isolation and partial characterization of antifungal compounds from Pseudomonas flourescens wild type

Date

4-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. Capareda

Abstract

RAROGAL, JOHN B., College of Arts and Sciences, University of the Philippines at Los Banos. Isolation and Partial Characterization of an Antifungal Compounds from Pseudomonas fluorescens wild type.

Adviser: Evamarie P. Capareda, PhD

Bioassay-guided flash column chromatography of the butanol extract from a still nutrient broth culture of Pseudomonas fluorescens wild type yielded a fraction At that was found to be active against Fusarium oxysporum, causal organism of Fuse/rim wilt in solanaceous crops. The compound gave a clear zone of 10 mm at 4000 ppm concentration.

Preliminary spectroscopic analysis (IR and 'H. NMR) indicated that fraction A I possibly has components with 0-H and aromatic groups. Reverse-phase 1IPLC of fraction Al on a RP-18 silica gel column using 70:30 acetonitrile-water as eluting solvent yielded three components. The major compound Al2 was active against Fusarium oxysporium, giving a clear zone of inhibition of 10 mm at 4000 ppm.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 C4 R37

Document Type

Thesis

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