Carica papaya latex (CPL) lipase-catalyzed interesterification of coconut oil

Date

10-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

REYES, JOSELETTE GREGORIO, College of Arts and Sciences, University of the Philippines at Los Bairns, October 1999. Carica papaya Latex (CPL) Lipase-Catalyzed lnteresterification of Coconut Oil.

Adviser: Dr. Eugenia M. Castillo

Carica papaya latex (CPL) lipase, an sn-3 stereoselective enzyme with a protein content of 0.42 mg/mL and a specific activity of 16.07 Units/mg protein was used to catalyze the interesterification of coconut oil. The reaction conditions were optimized by varying the incubation temperature and time of the system. Hydrolysis and interesterification reactions that occurred simultaneously were monitored by HPLC analysis of the interesterified glycerides and by GLC of liberated FFA.

Changes in concentration of triglyceride were noted. The highest concentration of triglyceride formed in the product was 28.29%. This was obtained when the system was incubated at 65°C for 12 hours. The fatty acid profile of structured lipids and physical blend showed that there was a 37.17 % increase in the oleic and linoleic acid content of structured lipids compared to that of the physical blend. An increase or decrease of these fatty acids during the preparation of structured lipids indicate that interesterification took place.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1999 C4 R69

Document Type

Thesis

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