Application of pectic enzyme of the extraction of essential oils from patchouli leaves (Pogostemon cablin)

Cyren M. Rico

Date

10-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Abstract

RICO, CYREN M., College of Arts and Sciences, University of the Philippines Los Banos, October 1999. Application of Pectic Enzyme on the Extraction of Essential Oils from Patchouli Leaves (Pogostemon cablin).

Adviser: DR. TERESITA M. ESPINO

Aqueous enzymatic extraction was investigated for the recovery of essential oil from Patchouli (Pogostemon cablin). The enzyme used in the study was pectinase with an average volumetric activity of 25.10 units per milliliter. The extracting solvent used was petroleum ether.

Several parameters such as enzyme concentration, incubation time, incubation temperature and buffer dilution ratio were optimized to increase the essential oil recovery. The optimized conditions were as follows: enzyme concentration = 1.0% (wlv) enzyme, incubation time = 8 hours, incubation temperature = 30°C, and sample:buffer dilution ratio = 1:2 (w/v). Using the optimized conditions. the highest percent oil recovery was 2.09% for the enzyme treated sample and 0.45% for the control.

The quality of enzyme extracted essential oil was better with respect to color and odor than the control and the commercially available essential oil imported from United States of America. The physico-chemical properties of the enzyme treated patchouli oil were as follows: specific gravity = 0.9548; refractive index = 1.39; acid value = 3.30; and solubility in 90% ethanol = 1 vol in 1 vol.

Extraction of essential oil from fresh patchouli leaves by pectic enzyme treatment showed significant improvement in both oil recovery and quality over the traditional steam distillation process.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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