Comparative analysis of essential oils from two accessions of patchouli Pogostemon cablin (Blanco) Benth.

Date

4-2007

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Hidelisa P. Hernandez

Abstract

The essential oils from the two accessions of patchouli (Pogostemon cablin (Blanco) Benth.) were extracted from the air-dried leaves using hydrosteam distillation. The range of % yield is 0.35 - 0.43 (v/w) and 0.75 - 2.53% (v/w) from the Faculty and Sta. Cruz I accessions, respectively. Both oils were clear yellow, VISCOUS liquid. The optical rotation of the oil from Faculty accession was - 44.88° while that of the Sta. Cniz 1 accession oil was - 56.78°. The solubility ratios of the oils of the oils expressed in oil:90% ethanol (w/v) are 1:2 for Faculty and 1:9 for Sta. Cruz I. The ester and acid values of the Faculty accession were determined to be 24.39 and 4.99, respectively. On the other hand, the Sta. Cniz 1 accession has an ester and acid value of 10.94 and 1.27, respectively.

The oils were subjected to thin layer chromatography to compare the chromatographic profiles of the oils from the two accessions. Ten spots were detected in the oil of the Faculty accession. O,te spot was unique to the Faculty accession since it is absent on the 5-month old and new oils of the Sta. Cruz 1 accession. Eight spots were detected in the 5-month old oil and six in the new Sta. Cruz 1 oil. The oil profile of the Sta. Cruz I changed with storage. One spot disappeared and three new spots were observed in the new and old oils, respectively. Probably, the lost spot in the new oil was converted to all or any one of the three compounds on the old oil.

Through gas chromatography-mass spectrometric analysis of the two oils, it was observed that the two oils were different with regards to the number of compounds and the concentration of the major components. Sixty five compounds were detected in the Faculty oil while only 41 compounds in the Sta. Cruz I oil. Thirty six compounds were common to both accessions. The major components which were common to both accessions were tentatively identified as patchouli alcohol, u- and fl-patchoulene, u- and P-caryophylIene, a-guaiene, u-bulnesene, and caryophyllene oxide. The first six major compounds were reported to be components of standard patchouli oil.

The results of this study suggest that the Sta. Cruz 1 accession has great potential for commercial production on the basis of its physico-chemical properties, oil yield, and oil composition. Based on its properties, Sta. Cruz I oil can be classified "special". i Thus, more research must he conducted for its utilization.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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