Optimization of conditions for laboratory scale acid protease production by solid substrate fermentation using Monascus anka Var. Rubellus 102 BIOTECH 3064

Date

4-1993

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Abstract

Optimization of conditions for acid protease production by solid substrate fermentation in the laboratory scale using Monascus mica var rubellus 102 BIOTECH 3064 was done. Optimum acid protease production was obtained in rice bran, with initial pH of 2-6 (0.025 M citrate - 0.050 M phosphate buffer), 5% (v/v) inoculum level, at 27 - 30 0 C (RT), and for an incubation time of 4 days. Comparison of acid protease production in 250 ml Erlenmeyer flasks and 3 1 enamelled trays by solid substrate fermentation was also done using the optimized conditions obtained. Results showed that acid protease activity was higher in 250 ml Erlenmeyer flasks than in 3 I enamelled trays (0.721 vs. 0.418 U/mg protein respectively).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

This document is currently not available here.

Share

COinS