Ultrafiltration of alpha-amylase and neutral protease as substitute to potassium bromate in bread making

Date

10-2002

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Abstract

SERRANO, BANYUHAY P. University of the Philippines Los Baftos, October 2002. Utilization of Alpha-Amylase and Neutral Protease as Substitute to Potassium Bromate in Breadmaking.

Adviser: Dr. Teresita M. Espino

Alpha-amylase and neutral protease were used as substitute for potassium bromate in producing pandesal (ordinary bread) of improved quality. These enzymes were obtained from the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos. Pandesal was prepared using the optimized concentrations of oc-amylase or neutral protease, separately and in combination; the best treatment for use as a supplement was evaluated. Consumer preference was determined by sensory evaluation method using the 7-point Hedonic scale.

Enzyme assays of a-amylase and protease showed activities of 2601.20 DUN/g and 19.41 units/g, respectively. The optimum concentration of a-amylase and neutral protease in the dough was each determined to be 0.010 %. Used singly, cc-amylase- and protease-supplemented pandesal were considered by panelists as having less desirable qualities than when used in combination. Alpha-amylase-supplemented pandesal had coarse and uneven crumb texture and perceived to be too sweet. An analysis of the sugar content of pandesal using the dinitrosalicylic acid (DNS) method confirmed this perceived quality. With neutral protease, the pandesal exhibited dark brown crust, greater volume and more compact texture. On the other hand, pandesal treated with a combination of both enzymes (0.005 % a-amylase and 0.005 % neutral protease) was most preferred by the panelists. This treatment produced pandesal with the most enhanced flavor, as well as, right bread softness and texture (compactness).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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