Evaluation of the envirologix cholinesterase screening test for organophosphate and carbamate pesticide residues in vegetables

Date

11-1998

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Amelia W. Tejada

Abstract

SUBSUBAN, JOSE RONELIO MATIAS. University of the Philippines at Los Banos, October, 1998. Evaluation of the EnviroLogix Cholinesterase Screening Test for Organophosphate and Carbamate Pesticide Residues in Vegetables.

ThesisAdviser: Dr. Amelia W. Tejada

The EnviroLogix Cholinesterase Screening Test, which involves acetylcholinesterase inhibition, was evaluated on different organophosphate and carbamate insecticides in vegetables. The insecticides were assayed in vitro by reacting with acetylcholinesterase in the presence of substrate acetylthiocholine. Percent inhibition was measured photometrically at 415 nm.

The cholinesterase screening test was found to be sensitive to a wide variety of organophosphate and carbamate pesticides. In eggplant samples, the order of sensitivity was : MIPC > chlorpyrifos > methamidophos > diazinon > malathion > and triazophos in the order of decreasing sensitivity. For pechay samples, the order of sensitivity was: chlorpyrifos > triazophos > methamidophos > malathion > MIPC > and diazinon while for eggplant samples, the trend was: MIPC > chlorpyrifos > triazophos > malathion > methamidophos > diazinon.

The method of extraction was validated on malathion by gas chromatography and this gave 96 percent recovery.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

UT

Document Type

Thesis

This document is currently not available here.

Share

COinS