Optimization of chitosan production from crab shell wastes and its use in wastewater treatment

Date

4-1998

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Veronica P. Migo

Abstract

JELYNNE PATERO TAMAYO. University of the Philippines Los Banos. Optimization of Chitosan Production from Crab Shell Wastes and Its Use in Wastewater Treatment.

Adviser: Dr. Veronica P. Migo

The conditions for effective chitosan production from crab shell wastes (carapace and appendages) were optimized. The optimal conditions for demineralization were 25% and 20% HCl at ambient temperature for carapace and appendages, respectively with a solid to solvent ratio of 1:6 (w/v). The optimal deproteination conditions involved treatment with 2.5% NaOH at 55°C for 5 hours with solid to solvent ratio of 1:6 (w/v). The chitin was satisfactorily deacetylated with 60% NaOH (w/v) under pressure for 30 minutes at 15 psi with a solid to solvent ratio of 1:6 (w/v). Percent deacetylation values were 75.68 and 65.28% for appendages and carapace, respectively and contained 5.10-5.79% crude nitrogen. The resulting chitosan was characterized in terms of viscosity, molecular weight, relative percent acetyl content, ash, nitrogen and IR analysis.

The chitosan produced from the crab shell wastes was evaluated for their decolorization efficiency. Comparing the two crab shell wastes, the chitosan obtained from appendages gave a high color reduction of 84% compared to 55% color reduction for carapace. The performance of chitosan as flocculent was evaluated in terms of pH, % decolorization and volume of sludge formed after flocculation.

Statistical analysis of data showed that there were significant effects due to concentration of HCl for demineralization. concentration of NaOH for deproteination and deacetylation as well as type of crab shell waste (carapace and appendages) at 5% level.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS