Purification of some Philippine plant viruses by the precipitation method

Date

11-1959

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Antonio D. Bustrillos

Abstract

PURIFICATION OF SOME PHILIPPINE PLANT VIRUSES BY THE PRECIPITATION METHOD

LUCIAN0 T. TALENS

ABSTRACT

Viruses can be precipitated and obtained in purified form by the chemical precipitation method. Tobacco mosaic, tomato mosaic, abaca mosaic, and abaca bunchy top virus were separated from the normal plant constituents by employing chemical precipitants such as 30% ammonium sulfate, zinc sulfate-copper sulfate (6.0-0.1), saturated calcium phosphate, and 0.1 M uranyl acetate.

Viruses precipitated with zinc sulfate-copper sulfate (6.0-0.1) gave purer virus isolates with minimum to undetectable amount of impurities than the other protein precipitants. Electron micrographs of the virus suspensions indicated evidence of micro impurities from about a micron to near virus size found associated with the suspected infectious entity. Occasional presence of slightly bigger particles was derived from the low centrifugation and partly, from the aggregation of finer particles. The effect of the individual protein precipitant upon the electron scattering property of the suspected virus particles in isolated form did not differ significantly. An increased contrast was indicated in the electron micrograph of the purified tobacco mosaic virus precipitated with uranyl acetate. this appeared to show that the action of the participant in enhancing the electron microscope images was specific.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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