Digital photometric analysis of reducing sugar in algal hydrolysate.

Date

5-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Charisse T. Reyes

Co-adviser

Ernesto J. Del Rosario

Abstract

Using digital photometric analysis, the reducing sugar content of hydrolysate was determined by 3,5-dinitrosalicylic acid assay. In this assay, the sugar reduces the organic DNS into 3-amino-5-nitrosalicylic acid. The amount of reduced DNS is proportional to the amount of glucose. Analysis by visible spectrophotometric method was compared with the digital photometric method to validate the results of digital photometry.

Digital photographs of the glucose solutions were taken using 12 MEGAPIXEL OLYMPUS FE-46 digital camera. The RGB Analysis of Image Colors software was used to gather RGB data from the photographs. R-G, % luminance, on and b* plots were generated in order to calculate the concentration of the hydrolysate sample. Using t-test, the concentration obtained from the spectrophotometric analysis was compared against concentrations obtained from different plots of digital photometry. The concentrations obtained from the spectrophotometric analysis and digital photometric analyses showed no significant difference at 95 % confidence level. R, 0, a*, b* and %luminance method of computing for the concentrations are then concluded to be comparable to that of the spectrophotometry analysis. The digital photometric determination of the concentration of the reducing sugar was reproducible and accurate. Hence, it can be an alternative method of determining reducing sugar concentration to the conventional visible spectrophotometric determination.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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