Evaluation of Celllulolytic Acitivity of Black Solider Fly (Hermetia illucens (Linneaus)) Larvae Fed with Different Combinations of Rice Bran and Municipal Organic Waste from Los Baños, Laguna

Date

6-2023

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Arvin Paul P. Tuano

Co-adviser

Amado A. Angeles

Committee Member

Val Jason G. Lagrada, Florinia E. Merca

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Request Access

To request access of this material, please email the administrator at uscs-mainlib.uplb@up.edu.ph

Abstract

Black soldier fly (BSF) larvae are widely used as live or processed organic feeds in poultry and fish production, aside from being popular biowaste decomposers. In this study. the growth rate and cellulolytic activity of BSF larvae were evaluated and compared using five combinations of rice bran (RB) and municipal organic waste (MW) as feeding/rearing substrates. The approximate growth rate of the BSF larvae was monitored from egg hatching until the 5th instar (harvest) stage. Crude protein, soluble protein, and cellulolytic activity were compared using 5th instar BSF larvae crude extracts. Moisture, crude protein. and crude fiber contents of the feeding substrates were also analyzed. Among all the treatments, BSF larvae fed with 50% RB: 50% MW exhibited the highest mean weight of pooled larvae (12.8 g), while those fed with 100% RB showed the lowest (8.7 g), also having the shortest rearing period (13 days). The longest rearing period was observed for those with 100% MW, lasting 21 days. The BSF larvae fed with 50% RB: 50% MW exhibited the fastest growth rate at 0.830 g/day, while those with 100% MW showed the slowest at 0.380 g/day, both measured at the final rearing phase before larvae harvest. The highest crude protein content (52.4%) was noted for the BSF larvae fed with 100% RB while the lowest (42.2%) was recorded for those fed with 100% MW, BSF larvae crude extracts had soluble protein contents ranging from 0.65-1.00 mg/mL. Crude extract from BSF larvae fed with 25% RB: 75% MW yielded the highest cellulolytic activity (0.465 µmol/min-mL), while the lowest was observed for those fed with 100% MW (0.172 µmol/min-mL). When treatments with combined RB and MW were compared, cellulolytic activity was found to increase with the soluble protein content of the BSF larvae, but both did not correlate with growth rate. The crude protein content of the feeding substrate was observed to be positively correlated with BSF larvae crude protein content and negatively correlated with total days of rearing, indicating that protein levels of feeding substrates may affect the rearing duration and nutrient content of BSF larvae intended for feed use.

Keywords: Black soldier fly larvae, cellulolytic activity, crude protein, municipal organic waste, rice bran

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2023 A13 A54

Document Type

Thesis

This document is currently not available here.

Share

COinS