Phenomics and Antimicrobial Activity of Actinobacteria Isolated from Bat Guano, Gut, and Rising Collected In and Around the Vicinity of Kamantigue Cave, Lobo, Batangas, Philippines

Date

6-2023

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Kristel Mae P. Oliveros

Co-adviser

Noel G. Sabino

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Abstract

Caves are environments that provide stable temperature and humidity to a wide variety of organisms. They are often known as shelter to bats that serve as hosts to different significant and pathogenic microorganisms. One of these significant microorganisms is the Actinobacteria, a robust source of commercially available antimicrobials. This makes caves as important sources of beneficial secondary metabolites. A total of 23 Actinobacteria isolates were obtained from three different sample sources (guano, gut, rinsing) collected from five species of bats (Taphozous melanopogon, Ptenochirus jagori, Hipposideros bicolor, H. antricola, and Rhinolophus arcuatus) caught inside and around the vicinity of Kamantigue Cave, Lobo, Batangas, Philippines. To determine the antimicrobial activities,the putative actinobacteria were tested against four bacterial pathogens using the Agar Plug and Agar Well Diffusion Assays. Isolates BRI-01 (8.64 mm) and 03 (12.39 mm) showed activity against Escherichia coli MCC MNH 1700, BRI-01 (44.06 mm) and 06 (12.45 mm) against Staphylococcus aureus MCC MNH 1702, BGA-01 (8.03 mm) and BRI-01 (7.18 mm) against Pseudomonas aeruginosa MCC MNH 1703,and BGA-02(11.66 mm) and BRI-01 (11.56 mm) against Shigella spp. MCC MNH 1704.Cultural, morphological, and biochemical characterization were done to identify the Actinobacteria isolates. This study confirms that cave bats are significant reservoirs of beneficial microorganisms which can be potential or alternative sources of antimicrobials for the emergence and possible re-emergence of infectious diseases.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2023 B4 A54

Document Type

Thesis

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