Alginate extraction and acid pretreatment of a brown alga (Sargassum sp. ) prior to saccharification and ethanol fermentation.

Date

3-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Co-adviser

Joy Elaine P. Kaligayahan

Abstract

WONG, RAYMART VILLENO. University of the Philippines at Los Baños, March 2011. Alginate Extraction and Acid Pretreatment of a Brown Alga (Sargassum sp.) Prior to Saccharification and Fermentation.

Adviser: Dr. Ernesto J. del Rosario Co-adviser: Prof. Joy Elaine P. Kaligayahan Advisory Committee Member: Dr. Norma N. Fajardo

Brown alga (Sargassum sp.) is a promising feedstock for ethanol production because of its high biomass productivity and favorable content of carbohydrates. The dilute sulfuric acid pretreatment of brown alga was conducted using the Box-Behnken design of Response Surface Methodology (RSM). The software StatEase Design Expert 8 generated the optimum conditions, namely 1% (v/v) H2SO4, 60 min at 60°C. Both pretreated and untreated algal samples were subjected to Simultaneous Saccharification and Fermentation (SSF). This involved saccharification of the sample at 50℃ for 24 h with a commercial enzyme preparation (Novozymes Cellic CTEc2 - cellulase and xylanase), followed by fermentation with Saccharomyces cerevisiae for 72 h at 30°℃. Both acid-pretreated and alginate-free algal samples gave a final ethanol concentration of 1.2 % (v/v) after 24 h of SSF. The saccharification approximately 78% and 84%, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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