Expression analyses of glutamate synthase and alcohol dehydrogenase genes in waterlogged corn (Zea mays L.) inbred Pi17

Date

6-2018

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Eureka Teresa M. Ocampo

Abstract

Induction of several genes in various crops exposed to waterlogging stress has been extensively studied. Long term exposure to waterlogging gives detrimental effects to plants, most especially non-tolerant species, since their normal metabolism is altered. In this study, corn (Zea mays L.) inbred Pi17 plants were exposed to waterlogging stress for 24, 48, and 72 hours. Morphologically, differences in root length and numbers were observed in non-waterlogged and waterlogged roots. Rapid increase in root length were observed and greater number of nodal roots and seminal roots were formed in waterlogged roots compared to the non-waterlogged. Moreover, leaf chlorosis, necrosis, and chlorotic leaf spots were observed in waterlogged roots due to the detrimental effects of long-term waterlogging exposure. Theoretically, these genes were induced upon exposure to the waterlogging so the induction and expression levels of glutamate synthase and alcohol dehydrogenase in Pi17, were analyzed using semi-quantitative PCR. Actual results showed that increased expression of the two genes were exhibited by Pi17 upon 24 hours exposure to waterlogging. However, decreased expression was observed from the two genes on longer exposure, such as 24 hours and 48 hours of waterlogging. Thus, induction of the glutamate synthase and alcohol dehydrogenase can serve as an adaptive mechanism of Pi17 to waterlogging stress but not for long-term exposure.

Language

English

LC Subject

Corn -- Effect of soil moisture on, Plant gene expression, Alcohol dehydrogenase, Insertional mutagenesis, Corn -- Mutation breeding |

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2018 A127 P365

Document Type

Thesis

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