Genetic diversity analysis of stingless bees, Trigona spp. (Hymenoptera: Apidea: Meliponini) from Mariveles, Bataan and Bulusan, Sorsogon based on COI gene

Date

4-2014

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ma. Carmina C. Manuel

Abstract

In order to assess the genetic diversity among stingless bees, a total of 18 colonies of Trigona sp. were sampled from Bataan and Sorsogon. DNA extraction was done on three adult worker bees from each colony. The mitochondrial cytochrome oxidase I (COI) gene was amplified from the total DNA extract. The amplicons were sequenced and analyzed. Using BLASTn, all the sequences obtained aligned best with the COI gene sequence of Melipona bicolor and Liotrigona sp. Tajima?s neutrality test showed that there are similar evolutionary rates present in the two localities. The phylogenetic tree based on the nucleotide consensus sequences of the samples showed that the colonies of Bataan clustered in one clade while the colonies of Sorsogon formed two distinct clades of Trigona sp., using Bombus terrestris as the outgroup. The obtained nucleotide sequence of Trigona sp. was computationally translated and aligned with the known amino acid sequence of M. bicolor. Graphical representation of the protein sequence alignment revealed that polypeptide binding domains, and ion binding domains were found in the amino acid sequence of Trigona and M. bicolor. The protein sequence-based maximum likelihood tree generated showed that the colonies of Bataan clustered into one clade while colonies of Sorsogon formed two distinct clades, which is similar to the phylogenetic tree based on nucleotide sequences. Diversity indices and genetic distances were computed using the Tamura 3-parameter model and revealed that there is little divergence within the colonies of Bataan and colonies of Sorsogon. The data gathered also showed that the bee populations from Bataan, and Sorsogon have a low interpopulational diversity index (d = 0.192) suggesting a high genetic similarity although variability was also present especially within the Sorsogon population.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 B4 /M68

Document Type

Thesis

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