Diversity analysis of abaca (Musa textilis Nee) accessions using microsatellite and evaluation for resistance to abaca bunchy top virus

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Fifty seven abaca accessions of the Institute of Plant Breeding were assessed using six SSR markers to determine their genetic diversity, identify resistant and susceptible accessions for bunchy top virus, and establish a basis for construction of a genetic base pool in breeding for resistance to bunchy top virus. Eighteen accessions were identified disease-free, twenty four accessions showed mild resistance, nine accessions were severely affected by Banana bunchy top virus (BBTV), and six accessions have no rating. All the accessions obtained from Palawan were observed as healthy. The 57 accessions are highly diverse with Shannon-Weaver index of 0.92 and polymorphism information content value was 0.61. They are grouped into seven distinct groups with 32.8% Jaccard?s similarity coefficient. Abuab Labo (resistant) and Maguindanao Pacol (resistant) have 56% and 45% similarity, respectively, to Pacol, a source of resistance to bunchy top. Principal component analysis displayed a widely diverse set of population where each resistant accession is distributed throughout the four panels.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 /B64

Document Type

Thesis

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