Serological and molecular detection, nucleotide sequence analysis and transmission of Banana bunchy top virus in abaca (Musa textilis Nee)

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Filomena C. Sta. Cruz

Abstract

Abaca bunchy top was first concluded to be caused by Banana bunchy top virus. However, conflicting evidence proposed that the disease is caused by a different species known as Abaca bunchy top virus. Thus, the identity of the virus causing abaca bunchy top remains to be ascertained. This study was conducted to determine the infectivity of BBTV in abaca through virus detection by serological and molecular methods, nucleotide sequence analysis and virus transmission test. A total of 56 abaca leaf samples from two locations in Leyte were found positive to BBTV by ELISA using the polyclonal BBTV antibody (Agdia) as capture antibody. BBTV was also detected by PCR using the primer pairs BBT-1 /BBT-2 and F3/ FPCR4 which amplified BBTV DNA-R, and the primer pairs G01/J02 and CBT3F.PRI/CBT3R.PRI which amplified the BBTV DNA-M and DNA-S components, respectively. BBTV detection by PCR using 1:20 diluted DNA template resulted in DNA amplification in more samples analyzed. The nucleotide sequences of PCR amplified viral DNA from abaca had 90-99% identity to the sequences of BBTV DNA components published in the NCBI Genbank and 97-99% identity with the virus detected in banana. BBTV was successfully transmitted to abaca using banana aphids and bunchy top symptoms, similar to those of inoculated banana, were observed in inoculated abaca at 2 months after inoculation. This study has demonstrated that BBTV can infect abaca and cause abaca bunchy top.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 /L59

Document Type

Thesis

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