DNA profiling of abaca (Musa textilis Nee) putative mutants derived from diethyl sulfate treatment for abaca bunchy top virus resistance

Date

7-2015

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

Seeds from Mapaso, Samoro and Laylay were treated with 2% Diethyl Sulfate (DES) for 24 hours (AM2) and 48 hours (BM2) with water as control. The 48 hour treatment was found to be more efficient in mutation breeding since it was able to produce more resistant mutants than the 24 hour treatment. A total of 43 abaca plants, including nine AM2 mutants, 31 BM2 mutants, two untreated plants and Pacol was screened using Musa¬-based SSR markers, resistant gene analogs (RGA) and host factor genes. Nine Musa-based SSR markers, four RGAs and 2 host factor genes were able to produce amplifications. Among the 15 loci, a total of 48 alleles with a mean value of 3.2 alleles per locus were generated. The banding patterns generated by Pacol, the source of resistance gene, were compared to the banding patterns produced by the mutants. The number of bands generated by the mutated accessions was found to be more diverse compared to the untreated. Thus, the markers used in the study can discriminate mutants from non-mutated genotypes and can further screen and select resistant from susceptible mutants. From the phenotypic and genotypic screening of the 40 abaca mutants tested, 15 were found to be resistant to bunchy top virus. These resistant abaca mutants could be used as another source of resistance gene that can be utilized in abaca varietal improvement programs.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A3 /R49

Document Type

Thesis

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