Isolation and sequence analysis of the large subunit ribosomal RNA of cashew (Anacardium occidentale L.) seeds
Date
7-2015
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Roberta N. Garcia
Abstract
Ribosomal RNA is the molecular form of prime importance in quantitatively analyzing evolutionary diversity at a molecular level, overpowering the morphological method often used by evolutionists. Successful RNA extraction from the Orange variety of cashew (Anacardium occidentale L.) was done using two different methods to see which would produce a better RNA to be used for the cDNA synthesis. Synthesis of cDNA was executed using the modified Superscript® III First-Strand Synthesis System. This was used as the template for PCR in amplifying the target gene. Optimization and adjustments in the PCR conditions were done to be able to hit the target gene. The product obtained was excised, purified and was sent for sequencing to AIT Biotech. The sequence result gave a result of 675 base pairs with no miscalled bases. After sequence analysis, the gene was highly homologous to the large subunit ribosomal RNA of various organisms of not less than 90%.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2015 A127 /R49
Recommended Citation
Reyes, Anna Clarisse Villacrusis, "Isolation and sequence analysis of the large subunit ribosomal RNA of cashew (Anacardium occidentale L.) seeds" (2015). Undergraduate Theses. 1496.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1496
Document Type
Thesis