Isolation and sequence analysis of the large subunit ribosomal RNA of cashew (Anacardium occidentale L.) seeds

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Roberta N. Garcia

Abstract

Ribosomal RNA is the molecular form of prime importance in quantitatively analyzing evolutionary diversity at a molecular level, overpowering the morphological method often used by evolutionists. Successful RNA extraction from the Orange variety of cashew (Anacardium occidentale L.) was done using two different methods to see which would produce a better RNA to be used for the cDNA synthesis. Synthesis of cDNA was executed using the modified Superscript® III First-Strand Synthesis System. This was used as the template for PCR in amplifying the target gene. Optimization and adjustments in the PCR conditions were done to be able to hit the target gene. The product obtained was excised, purified and was sent for sequencing to AIT Biotech. The sequence result gave a result of 675 base pairs with no miscalled bases. After sequence analysis, the gene was highly homologous to the large subunit ribosomal RNA of various organisms of not less than 90%.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /R49

Document Type

Thesis

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