Molecular characterization of taro (Colocasia esculenta (L.) Schott) using microsatellite markers
Date
12-2014
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Antonio G. Lalusin
Abstract
DNA isolation and Simple Sequence Repeats Polymerase Chain Reaction (SSR-PCR) were optimized on 46 accessions of taro (Colocasia esculenta (L.) Schott) obtained from National Plant Genetic Resources Laboratory, Institute of Plant Breeding, Crop Science Cluster using 15 primer pairs from three crop-based markers. Three protocols were tested for optimization of DNA isolation of taro. DNA extraction method for taro [1] yielded the best quality and high purity DNA which was used for SSR diversity analysis. Of the 15 primer pairs used in PCR, only 10 were optimized. The optimized protocol used 30 ng and 60 ng DNA template, 0.05 units Taq polymerase and 7 µL reaction mixtures. Resulting banding patterns for the 10 SSR markers ranged from 1 to 39 with Polymorphic Information Content (PIC) ranging from 0.69 to 0.96 which indicates high genetic diversity. Cluster analysis using Jaccard's coefficient at 0.72 generated 8 clusters which does not show association between geographic origin and genotypes of germplasm resources.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2014 A127 /R37
Recommended Citation
Rasco, Jhun Laurence Samosa, "Molecular characterization of taro (Colocasia esculenta (L.) Schott) using microsatellite markers" (2014). Undergraduate Theses. 1495.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1495
Document Type
Thesis