Molecular characterization of taro (Colocasia esculenta (L.) Schott) using microsatellite markers

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

DNA isolation and Simple Sequence Repeats Polymerase Chain Reaction (SSR-PCR) were optimized on 46 accessions of taro (Colocasia esculenta (L.) Schott) obtained from National Plant Genetic Resources Laboratory, Institute of Plant Breeding, Crop Science Cluster using 15 primer pairs from three crop-based markers. Three protocols were tested for optimization of DNA isolation of taro. DNA extraction method for taro [1] yielded the best quality and high purity DNA which was used for SSR diversity analysis. Of the 15 primer pairs used in PCR, only 10 were optimized. The optimized protocol used 30 ng and 60 ng DNA template, 0.05 units Taq polymerase and 7 µL reaction mixtures. Resulting banding patterns for the 10 SSR markers ranged from 1 to 39 with Polymorphic Information Content (PIC) ranging from 0.69 to 0.96 which indicates high genetic diversity. Cluster analysis using Jaccard's coefficient at 0.72 generated 8 clusters which does not show association between geographic origin and genotypes of germplasm resources.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /R37

Document Type

Thesis

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