Fungi causing fruit rot of roseleaf raspberry (Rubus rosifolius Sm.) in the Philippines
Date
6-2015
Degree
Bachelor of Science in Agriculture
Major Course
Major in Plant Pathology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Teresita U. Dalisay
Abstract
Eight fungal genera were proved to be the causal organisms of roseleaf raspberry fruit rot upon conduct of the pathogenicity test. The degree of rot in relation to the absence or presence of scanty to profuse mycelial growth determined the virulence of the fungal isolates. The above criteria for evaluation utilized the Visual Quality Rating Scale (VQRS). Among the isolates, sterile mycelia obtained the highest VQRS of 2.22 which caused moderate rotted tissues and dense mycelial growth followed by Fusarium sp. AFAB7 (1.89) and Fusarium sp. AFAB 8 (1.78). A little over and mid-way between 1 and 2 VQRS was manifested by Pestalotiopsis sp. AFAB 2 (1.44), Fusarium sp. AFAB 4 and AFAB 9 (both with VQRS of 1.22), and Phoma sp. AFAB 6 (1.11). Least capacity to cause infection was coelomycete sp. AFAB 5 with VQRS of 0.89. Cultural characterization revealed that Pestalotiopsis sp. AFAB 2 grew best on MEA and PDA; sterile mycelia AFAB 3 on PDA; Fusarium sp. AFAB 4 on MEA, RLDA and PDA; coelomycete sp. AFAB 5 on PDA, RLDA and RSDA; Phoma sp. AFAB 6 on RLDA and PDA,; Fusarium sp. AFAB 7 on PDA, MEA and RLDA,; Fusarium sp. AFAB 8 on all the media and Fusarium sp. AFAB 9 on PDA, MEA, RLDA and RSDA. Different light and dark conditions gave unpredictable patterns of growth on the isolates but generally, all the isolates gained their highest peak of growth on the first day after mycelial disc transfer. The growth rate and colony growth of each isolate were almost statistically the same. Moreover, the effect of different light and dark conditions was evident on coelomycete AFAB 5 which had the best growth upon exposure to total darkness. Results showed that the optimum temperature that favored the growth of the fungal isolates ranges from 24°C to 27°C. Higher temperatures 30°C to 37°C had consistently inhibited the growth of the isolates.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2015 A3 /J35
Recommended Citation
Jain, Llewelyne Caro, "Fungi causing fruit rot of roseleaf raspberry (Rubus rosifolius Sm.) in the Philippines" (2015). Undergraduate Theses. 1543.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1543
Document Type
Thesis