Effect of priming on morphology and amylase activity of rice (Oryza sativa L.) genotypes varying in submergence tolerance during germination

Date

4-2010

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maribel L. Dionisio-Sese

Co-adviser

Abdelbagi M. Ismail

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Abstract

Submergence tolerance during rice (Oryza sativa L.) germination is an important trait required for direct seeding, a method of crop establishment which is becoming popular in response to the looming scarcity of crop land, laborers and water. Tolerance to flooding during germination is limited, however, to a small number of genotypes. Breeding cultivars with anaerobic germinability (AG) and application of seed invigoration techniques, such as seed priming, are approaches on improving germination under flooded conditions. The effect of priming on morphology was observed on four groups from 21 rice accessions (AG-parental, AG-derived, Non-AG non-sub1, Non-AG sub1) under laboratory (deoxygenated stagnant agar) and greenhouse (flooded soil) conditions. Under laboratory condition, varying the priming medium and its duration showed that 24h:24h (soaking:drying) hydropriming significantly improved germination and coleoptile length especially in the intolerant lines. This priming method also improved survival, coleoptile extension, mesocotyl length and root growth under greenhouse condition. Comparison of germination in greenhouse and laboratory conditions showed that the positive effect of priming was greater in hypoxic agar while enhancement of coleoptile length was manifested more in flooded soil. Total amylase activity which was attributed more to α-amylase than β-amylase, was observed higher among AG-parental lines, although soluble sugar concentration was similar in all the genotypes tested. Priming has an overall positive effect on both amylase activity and soluble sugar concentration. Transcript profile analysis of several fermentative enzyme coding-genes revealed no significant difference between representative AG-parental, AG- derived and intolerant lines under hypoxia.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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