Association of putative viral RNA sequences to flower color variegation in Japanese primrose (Primula sieboldii E. Morren) cultivars

Date

5-2015

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

Flower color variegation is characteristic symptom of viral infections in ornamentals such in the case of camellia, tulips, petunia, and daffodil. This is also seen in different cultivars of Japanese primrose like Shichikenjin, Sotorihime and Isaribi. Putative viral RNA sequences were first identified in the Primula sieboldii databases. Total RNA was then isolated and reverse transcriptase polymerase chain reaction was done using virus-specific primers to identify the association between the presence of putative viral RNA sequences and flower color variegation. Seven putative viral RNA homologous sequence groups were identified, with a possible virus identity of Cycas necrotic stunt virus, Lettuce big-vein associated virus and Rosa multiflora cryptic virus. Results showed that instances of amplifications were seen in both variegated and nonvariegated within and across cultivars. This denotes the presence of putative viral RNA and a possible viral morbidity in the amplified cultivars. Furthermore, instances wherein amplification of non-variegated flowers while no amplification of variegated flowers within the same cultivar were also observed. Variegated plants of cultivarswhich contain variegated and non-variegated flowershad a weak correlation. Thus, no definite association could be deduced from the presence of putative viral RNA and flower color variegation. It is also recommended that flower tissue should be used for gene expression analysis to avoid error in differential gene expression.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A3 /G46

Notes

NO ABSTRACT

Document Type

Thesis

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