Response of mixed cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) on salicylic acid treated mokara protocorms in-vitro and their transmission and movement in cultivated dendrobium UH hybrid

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Evalour T. Aspuria

Abstract

The research internship was conducted in Orchid Crop Nursery, Tissue Culture Facility of the Crop Science Cluster and Host-Pathogen Interaction Laboratory of the Crop Protection Cluster from May 2014 to June 2015. The internship includes elucidation of the effect of varying concentration of salicylic acid on in-vitro derived Mokara protocorms infected with mixed infection Cymbidium mosaic potexvirus and Odontoglossum ringspot tobamovirus and determination of the rate of movement of the mixed virus in cultivated Dendrobium UH. Four treatments of varying concentrations of salicylic acid (SA) (0, 8, 16 and 32 mg/L) in ½ MS medium supplemented with 1 mg/L Benzyladenine, 20% coconut water were used to evaluate the effect of salicylic acid on virus resistance protocorms infected with mixed CymMV and ORSV. At higher concentration of SA, growth inhibition as well as browning was seen in the protocorms. Using Indirect ELISA, results showed that salicylic acid has minimal effect in plant resistance against the mixed virus. Protocorms treated with 8mg/L SA was able to produce virus-free plants with 15% efficacy while treatment 4 with 32mg/L had 5% efficacy at 30 days after inoculation. In another experiment, the rate of translocation and movement of both viruses as mixed infection was evaluated in Dendrobium UH hybrids under greenhouse conditions. The target viruses were detected in various plant organs using I-ELISA and results showed that the viruses were translocated completely to the whole plant at 21 DAI.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /A43

Notes

Major in Crop Biotechnology

Document Type

Thesis

This document is currently not available here.

Share

COinS