Development of molecular markers tagging resistance genes for abaca bunchy top virus and fiber quality in abaca (Musa textilis Nee)
Date
7-2015
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Antonio G. Lalusin
Abstract
The study was conducted to develop molecular markers tagging resistance genes for Abaca Bunchy Top Virus and fiber quality in abaca. A total of thirty-one primers were screened for differential band amplification. Out of these primers, four primers (mMaCIR264, RGA1, mMaCIR150, and pMaCIR348b) amplified four unique bands on Abuab, Pacol and Inosa. These bands were sequenced and primers were developed from the resulting sequences. Six primer pairs were designed (MtNBS-LRRP1, MtNBS-LRRP2, MbP1, MbP2, 348bI1, and 348bI2). After validation of primers, three were tagged as non-specific and three were gene specific (MbP1, 348bI1, and 348bI2). MbP1 was associated to Bunchy Top Virus resistance whereas 348bI1 and 348b2 were associated to tensile strength of fiber.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2015 A127 /A73
Recommended Citation
Aragon, Ralph Quizon, "Development of molecular markers tagging resistance genes for abaca bunchy top virus and fiber quality in abaca (Musa textilis Nee)" (2015). Undergraduate Theses. 1607.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1607
Document Type
Thesis
Notes
Major in Crop Biotechnology