Development of molecular markers tagging resistance genes for abaca bunchy top virus and fiber quality in abaca (Musa textilis Nee)

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

The study was conducted to develop molecular markers tagging resistance genes for Abaca Bunchy Top Virus and fiber quality in abaca. A total of thirty-one primers were screened for differential band amplification. Out of these primers, four primers (mMaCIR264, RGA1, mMaCIR150, and pMaCIR348b) amplified four unique bands on Abuab, Pacol and Inosa. These bands were sequenced and primers were developed from the resulting sequences. Six primer pairs were designed (MtNBS-LRRP1, MtNBS-LRRP2, MbP1, MbP2, 348bI1, and 348bI2). After validation of primers, three were tagged as non-specific and three were gene specific (MbP1, 348bI1, and 348bI2). MbP1 was associated to Bunchy Top Virus resistance whereas 348bI1 and 348b2 were associated to tensile strength of fiber.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /A73

Notes

Major in Crop Biotechnology

Document Type

Thesis

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