A research internship on SSR polymorphism analysis between coconut parental genotypes and morphological characterization of selected coconut palms

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Alma O. Canama

Abstract

Basic molecular biology techniques and skills including DNA extraction, agarose gel electrophoresis, polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis were properly conducted and applied to coconut simple sequence repeat (SSR) DNA polymorphism analysis. DNA extraction of coconut parental genotypes using three modified DNA isolation protocols based on Doyle and Doyle (1990) was successfully carried out and lyophilization of leaf samples prior to DNA extraction yield the best quality and quantity of genomic DNA. For SSR polymorphism analysis, a total of 16 SSR coconut primers were used to identify polymorphic markers between coconut parental genotypes. Of the 16 primers, 14 were polymorphic and two (2) were monomorphic. Coconut parental genotypes Catigan Dwarf (CATD) and N-18, CATD and CW-29, and CATD and AN-14 revealed the highest percent polymorphism of 81% while CATD and Laguna Tall (LAGT) parental genotypes obtained the lowest percent polymorphism (50%). High DNA polymorphism between the coconut parental genotypes indicated high genetic variation among the coconut genotypes. A total of 32 characters were observed among the ten (10) randomly selected coconut palms and there is slight variation among the plant, leaf, fruit and nut characters measured. Evaluation of additional coconut palms give a better measure of morphological variation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2014 A127 /I83

Notes

major: Crop Biotechnology

Document Type

Thesis

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