Isolation and screening of phytate-degrading bacteria from the Philippine soil using Okara as substrate Zyrine Celis Lorizo

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lotis Escobin-Mopera

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Phytate-degrading bacteria from the Philippine soil using Okara as a substrate were isolated. Soil samples from a ?taho?-making plant in Laguna (S1) and garden soil (S2) from around Food Science Cluster were collected as putative sources of phytate-degrading bacteria. A total of 17 isolates were screened for their phytase activity. The two highest extracellular and intracellular phytase producing isolates were identified using biochemical tests. The extracellular and intracellular phytase producing bacteria were identified as Bacillus azotoformans (3.24 U/mg) and Klebsiella pneumoniae (2.17 U/mg). As a preliminary study, the effect of different pH and temperature on phytase activity were determined. Extracellular phytase activity of B. azotoformans was observed at pH 7.6 On the other hand, K. pneumoniae showed the highest phytase activity on at pH 6. Intracellular phytase activity for both isolates were observed. However, K. pneumoniae produced a more stable activity than B. azotoformans. Results also showed that at 45oC, both isolates exhibited intracellular and extracellular phytase activity. Results showed that there is no significant difference in the intracellular and extracellular phytase activity of when both isolates were grown in different pH and temperature Further study is recommended to explore the phytate degrading abilities of the two isolates.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /L67

Notes

Major: Food Biotechnology

Document Type

Thesis

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