Comparative protein profiling and proteolytic activity of crude duodenal proteases from broilers fed avilamycin and varying levels of a benzoic acid and essential oils mixture Jeremae Barias Manalaysay

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

To compare the protein profile and the proteolytic activity of the crude duodenal proteases from broilers fed Avilamycin and varying concentrations of a mixture of benzoic acid and essential oil compounds (BA/EO), 20 day-old broilers were divided into 4 groups and assigned to 4 treatments, specifically, a basal diet, basal diet + 10 ppm Avilamycin, basal diet + 150 ppm BA/EO, and basal diet + 300 ppm BA/EO, following Completely Randomized Design (CRD). All growth parameters were recorded. After slaughter, duodenal contents were collected and subjected to proteolytic assays. Subsequently, the protein profiles of the crude duodenal contents were determined using SDS-PAGE. Results showed no significant differences on the growth performance, and duodenal proteolytic activity of the broilers fed with the different treatments (p>0.05). The electrophoretogram showed qualitative differences in band intensity of the enzymes trypsin, chymotrypsin, elastase, carboxypeptidase, and two subunits of aminopeptidase. Under the conditions on which this study was conducted, it can be said that inclusions of Avilamycin, and also 150 ppm and 300 ppm of BA/EO caused qualitative differences in the protein profile of the broilers? duodenal contents but these deviations were not enough to affect the animal?s growth performance and duodenal proteolytic activity.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /M35

Notes

Major: Animal Biotechnology

Document Type

Thesis

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