Comparative analysis of Philippine native chicken (Gallus gallus domesticus) banaba genetic group and commercial broiler chicken cecum microbial communities using denaturing gradient gel electrophoresis / John Frederick Ramilo Manalo Medino Gedeun N. Yebron, Jr., adviser.

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Medino Gedeun N. Yebron, Jr.

Abstract

This study aims to characterize and compare the microbial communities of the two chicken groups using Denaturing Gradient Gel Electrophoresis (DGGE). DNA extraction was done using two protocols zirconia beads extraction and phenol-chloroform extraction. PCR amplification was done using V3 DGGE primers wherein the PCR products obtained were subjected to DGGE. Results showed that the commercial broiler chicken group exhibited an average of ten bacterial species per chicken and having a total of seventeen bacterial species detected as a group whereas the native chicken group exhibited an average of twelve bacterial species per chicken and having a total of 21 bacterial species detected as a group. Among identified bacterial species were Clostridium hylemonae which belonged to the Proteobacteria Phylum while Barnesiella viscericola and Bacteroidetes caecigallinarum sp. both belonged to the Bacteroidetes Phylum. Others were found to be uncultured bacteria belonging to the Firmicutes, Bacteroidetes and Unclassified Bacteria Phyla. Feed additives, litter composition and other factors of the commercial broiler chicken group may have played a factor in enhancing its bacterial ecology by increasing the dominance of some bacterial species resulting for the samples to exhibit distinct bands compared to the native chicken group.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /M36

Notes

Major: Animal Biotechnology

Document Type

Thesis

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