Lactase (ß-galactosidase) in buffalo's (Bubalus bubalis L.) milk inoculated with Aspergillus oryzae (Ahlb.)

Date

7-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Maria Cynthia R. Oliveros

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

To detect the presence of lactase (β-galactosidase) in buffalo's milk after inoculation of Aspergillus oryzae L., 100 ml whole buffalo's milk was inoculated with 10 ml spore suspension (1.12 x 107 spores/ml) and another 100 ml was added with supernatant from previously inoculated milk. These were then incubated for 4 hours at 30 °C. Results showed significant differences between mean lactose contents and reducing sugar contents (P𕟨.05). The lactose content and reducing sugar content decreased after addition of A. oryzae L.. The lactose content of whole buffalo's milk decreased after addition of supernatant from a previously inoculated milk while the reducing sugars increased. The reduction in lactose and reducing sugars after inoculation of A. oryzae L. in milk was due to the utilization of fungal spores to lactose as carbon source for lactase production. The reduction of lactose content and increment in reducing sugars after addition of supernatant from previously-inoculated milk into another set of whole buffalo's milk was due to enzymatic action of extracellular lactase produced by the Aspergillus oryzae L.. The study adopted submerged fermentation which did not allow maximum production of lactase. It was then recommended that longer incubation period be studied. The study therefore indicates the potential of Aspergillus oryzae L. to produce lactase which is an important enzyme in dairy industry.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2014 A3 /D45

Document Type

Thesis

This document is currently not available here.

Share

COinS