Identification of the pathogen associated with the TIP-MELT Disease of impatiens(Impatiens Wallerana hook f.)

Date

2011

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

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Abstract

A fungus with hyaline, septated and branched mycelia was isolated from diseased impatiens (Impatiens wallerana Hook F.). This was grown into pure culture and was inoculated to healthy impatiens plants. After the expected symptoms were observed, the pathogen was re–isolated in vitro. This was identified and characterized to determine the pathogen associated with the tip–melt disease of impatiens. Profuse white, fluffy and branched mycelia were observed. The length of the cells ranged from 1.65μm to 2.7μm (x=2.15μm ± 0.32μm) when measured from one cross wall to another. The degree of branching resembled an acute angle. The pathogen was identified as Sclerotium rolfsii Sacc. belonging to the Mycelia Sterilia with no sexual stage. The pathogen favored a mycelia growth temperature of about 25°C - 30°C. Acidic pH 5 and continuous dark condition induced formation of round and compact brown to black sclerotial bodies. Optimum mycelia growth was observed at pH 5, 30°C and continuous dark condition. Formation of mycelia did not always lead to the production of sclerotia. Some formed abundant mycelia but were not able to produce sclerotia.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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