Percentage of recurrent allele in anaerobic germination (AG2) introgression lines of rice (Oryza sativa L.)

Date

4-2014

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Indang M. Septiningsih

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

To assess the percentage of recurrent alleles in AG2 introgression lines of rice (Oryza sativa L.), a cross between IR42 (a mega variety susceptible to Anaerobic Germination) and Ma-Zhan Red (a wild type variety tolerant to Anaerobic Germination) was first performed in previous studies to identify QTLs responsible for the AG-tolerance in rice. The F3:2 generation was crossed with mega varieties (IR64 and IR64-Sub1), which served as the recurrent parent, to transfer the AG-tolerant allele. The progenies were backcrossed to the recurrent parent until the BC4F2 generation of IR64 and BC3F2 generation of IR64 were derived producing the AG2 introgression lines. The introgression lines were genotyped using 54 SSR markers covering the 12 chromosomes. Analysis of the genotypic data was done using Graphical Genotyping (GGT32) software to visualize the chromosomes of the introgression lines. In BC4F2 generation of IR64, the percent recurrent allele was 83.333% while in the BC3F2 generation of IR64-Sub1, it was 74.07%. Since the percent recurrent allele of the mega varieties are lower than 95%, they are not yet suitable for further testing in other environments. Further backcrossing must be done to recover all the potential alleles of the recurrent parent.

Language

English

Call Number

LG 993.5 2014 B4 /T87

Notes

Major in Cell and Molecular Biology

status: in process

loc: UPLB Main Library Cataloging Section

Document Type

Thesis

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