Characterization of Ascosphaera apis isolated from chalkbrood infected colonies of European honeybee, Apis Mellifera Linnaeus and its susceptibility to sodium benzoate and calcium propionate
Date
4-2014
Degree
Bachelor of Science in Biology
Major Course
Major in Microbiology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Renard M. Jamora
Restrictions
Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.
Abstract
Different apiaries of Apis mellifera were surveyed in Batangas for Chalkbrood infection by fungus Ascosphaera apis. Three apiaries were positive for Chalkbrood. Symptoms include white-cotton-like mycelia growing on the larvae resulting to chalk-like mummies. Mummified larvae were collected and isolates were grown on Potato Dextrose Agar (PDA) with 100 ppm Streptomycin. Isolates were described based on cultural and morphological characteristics, growth rate and ascoma size. The three isolates and the stock culture produced typical Ascosphaera apis colonies: white mycelium at the center surrounded by black to grey ring of sporulating mycelia then white mycelia again on the edges. A variant of this culture has thick white mycelia with few black spores beneath. Growth rates increase steadily for the first four days of incubation then slow down during sporulation and increase again afterwards. Microscopically, the isolates produce dichotomously branched hyphae with brown mature ascoma. The ascoma sizes are relatively small indicating that the isolates belong to the minor variety of Ascosphaera. For the cylinder cup assay, no zones of inhibition were observed for Sodium benzoate and Calcium Propionate indicating their ineffectivity in controlling Chalkbrood. Previous studies suggest the presence of symbiotic fungi rendering Ascosphaera insensitive to the test substances.
Language
English
Call Number
LG 993.5 2014 B4 /S26
Recommended Citation
Sangel, Alyxandra Kim A., "Characterization of Ascosphaera apis isolated from chalkbrood infected colonies of European honeybee, Apis Mellifera Linnaeus and its susceptibility to sodium benzoate and calcium propionate" (2014). Undergraduate Theses. 1965.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1965
Document Type
Thesis
Notes
status: in process
loc: UPLB Main Library Cataloging Section