Characterization of Ascosphaera apis isolated from chalkbrood infected colonies of European honeybee, Apis Mellifera Linnaeus and its susceptibility to sodium benzoate and calcium propionate

Date

4-2014

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Renard M. Jamora

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Different apiaries of Apis mellifera were surveyed in Batangas for Chalkbrood infection by fungus Ascosphaera apis. Three apiaries were positive for Chalkbrood. Symptoms include white-cotton-like mycelia growing on the larvae resulting to chalk-like mummies. Mummified larvae were collected and isolates were grown on Potato Dextrose Agar (PDA) with 100 ppm Streptomycin. Isolates were described based on cultural and morphological characteristics, growth rate and ascoma size. The three isolates and the stock culture produced typical Ascosphaera apis colonies: white mycelium at the center surrounded by black to grey ring of sporulating mycelia then white mycelia again on the edges. A variant of this culture has thick white mycelia with few black spores beneath. Growth rates increase steadily for the first four days of incubation then slow down during sporulation and increase again afterwards. Microscopically, the isolates produce dichotomously branched hyphae with brown mature ascoma. The ascoma sizes are relatively small indicating that the isolates belong to the minor variety of Ascosphaera. For the cylinder cup assay, no zones of inhibition were observed for Sodium benzoate and Calcium Propionate indicating their ineffectivity in controlling Chalkbrood. Previous studies suggest the presence of symbiotic fungi rendering Ascosphaera insensitive to the test substances.

Language

English

Call Number

LG 993.5 2014 B4 /S26

Notes

status: in process

loc: UPLB Main Library Cataloging Section

Document Type

Thesis

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