Identification of chromosome segments from Hasawi associated with salt tolerance of rice (Oryza sativa L.) in BC4F4 population using single nucleotide polymorphisms (SNPs)

Date

4-2014

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn O. Villa

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Chromosome segments from Hasawi associated with salt tolerance of rice in BC4F4 population were identified using single nucleotide polymorphisms (SNPs). Morphological and physiological parameters were used to construct a linkage map and identify QTLs. When the 29 genotypes were screened for tolerance, twelve genotypes were tolerant, two were susceptible and fifteen genotypes were moderately tolerant under EC 12 screening. Based on the evaluation of their morphological traits, the mean values of the progenies were close to the mid-parental values suggesting the contribution of Hasawi and Ciherang for the expression of the parameters. Physiological measurements showed that higher Na+/K+ ratios were obtained when the plants were subjected to EC 18 salt stress level than when subjected to EC 12. Molecular genotyping revealed 1210 polymorphic SNP markers. A total of 30 segments introgressed from Hasawi was present in the tolerant and moderately tolerant genotypes but absent in the two susceptible lines. QTLs with LOD scores > 3.0 were found and four out of 22 significant QTLs associated to salt tolerance were confirmed. The QTLs found in chromosome 3 were associated with reduced shoot length.

Language

English

Call Number

LG 993.5 2014 B4 /R65

Notes

Major in Genetics

status: in process

loc: UPLB Main Library Cataloging Section

Document Type

Thesis

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